tissue microarrays technology - PataGen Inc.

A
pplications

¡æ Tissue microarray technology, which was first used in published research by J. Kononen et al. (Nature Medicine Vol 4. No. 7, 1998 pp. 844-847), has been drawing a great deal of attention recently from a wide variety of research interests. The technology itself is very similar to other high throughput systems such as DNA microarray, protein array etc. from several ways(Please, see the ¡°Tissue Microarray¡± on this website)
.

The technology enhances the productivity of drug development by shortening target validation and allowing to check the cross reactivity of a therapeutic antibody candidate in a very short period of time. In addition to these commercial advantages, many academic researchers can enhance their research performance in the various fields of basic life science, especially medical science.

¡æ Typical examples of possible applications

Validation of targets/markers Protein work via
immuno-
histochemistry

 

Expression pattern of a target 1),2),7) * in every kind
   of Cancer
   tissue
* in various
  related   diseases

  6)
, 7)
* during the   carcinogenesis   process

   2)
,4),5),10)
* during
   metastasis of
   tumors
Streamlined application of the results of animal model or cell line studies to human tissue

 

DNA via FISH 3)

 

RNA via ISH

 

Preclinical development of therapeutic candidates Cross reactivity of an antibody candidate

 



1) American Journal of Pathology 2001 158: 1335-1344.
Expression and Potential Role of Fas-Associated Phosphatase-1 in Ovarian Cancer

Ivo Meinhold-Heerlein, Frank Stenner-Liewen, Heike Liewen, Shinichi Kitada, Maryla Krajewska, Stanislaw Krajewski, Juan M. Zapata, Anne Monks, Dominic A. Scudiero, Thomas Bauknecht, and John C. Reed

2) The American Journal of Surgical Pathology 2002 26:312-319

Tissue Microarray Sampling Strategy for Prostate Cancer Biomarker Analysis

Mark A. Rubin, M.D.; Rodney Dunn, M.S.; Myla Strawderman, M.S.; Kenneth J. Pienta, M.D.

3) Cancer Research 2002 62: 1256-1260.
Clinical Validation of Candidate Genes Associated with Prostate Cancer Progression in the CWR22 Model System using Tissue Microarrays

Spyro Mousses, Lukas Bubendorf, Urs Wagner, Galen Hostetter, Juha Kononen, Robert Cornelison, Natalie Goldberger, Abdel G. Elkahloun, Niels Willi, Pasi Koivisto, William Ferhle, Mark Raffeld, Guito Sauter, and Olli-P Kallioniemi


4) Cancer Research 1999 59: 803-806.
High-Throughput Tissue Microarray Analysis of 3p25 (RAF1) and 8p12 (FGFR1) Copy Number Alterations in Urinary Bladder Cancer

Ronald Simon, Jan Richter, Urs Wagner, Andre Fijan, James Bruderer, Ulrico Schmid, Daniel Ackermann, Robert Maurer, Goran Alund, Hartmut Knonagel, Marcus Rist, Kim Wilber, Manuel Anabitarte, Franz Hering, Thomas Hardmeier, Andreas Schonenberger, Renata Flury, Peter Jager, Jean Luc Fehr, Peter Schraml, Holger Moch, Michael J. Mihatsch, Thomas Gasser, and Guido Sauter

5) Cancer Research 2001 61: 4514-4519.
High-Throughput Tissue Microarray Analysis of Cyclin E Gene Amplification and Overexpression in Urinary Bladder Cancer

Jan Richter, Urs Wagner, Juha Kononen, Andre Fijan, James Bruderer, Ulrico Schmid, Daniel Ackermann, Robert Maurer, Goran Alund, Hartmut Knonagel, Marcus Rist, Kim Wilber, Manuel Anabitarte, Franz Hering, Thomas Hardmeier, Andreas Schonenberger, Renata Flury, Peter Jager, Jean Luc Fehr, Peter Schraml, Holger Moch, Michael J. Mihatsch, Thomas Gasser, Olli P. Kallioniemi, and Guido Sauter

6) The Journal of Pathology 2001 194 (3): 349-357
Microarrays of bladder cancer tissue are highly representative of proliferation index and histological grade

Antonio Nocito, Lukas Bubendorf, Eva Maria Tinner, Katrin Suess, Urs Wagner, Thomas Forster, Juha Kononen, Andre Fijan, James Bruderer, Ulrico Schmid, Daniel Ackermann, Robert Maurer, Goran Alund, Hartmut Knonagel, Marcus Rist, Manuel Anabitarte, Franz Hering, Thomas Hardmeier, Andreas J. Schoenenberger, Renata Flury, Peter Jager, Jean Luc Fehr, Peter Schraml, Holger Moch, Michael J. Mihatsch, Thomas Gasser, Guido Sauter

7) American Journal of Pathology 1999 154: 981-986.
Identification of Differentially Expressed Genes in Human Gliomas by DNA Microarray and Tissue Chip Techniques

Satu-Leena Sallinen, Pauli K. Sallinen, Hannu K. Haapasalo, Heikki J. Helin, Pauli T. Helen, Peter Schraml, Olli-P. Kallioniemi, and Juha Kononen

8) Applied Immunohistochemistry & Molecular Morphology 2001 9:358-363
Tissue Microarray Analysis of ß-Catenin in Colorectal Cancer Shows Nuclear Phospho-ß-catenin Is Associated with a Better Prognosis

Gina G. Chung, Elayne Provost, Eric P. Kielhorn, Lori A. Charette, Bradley L. Smith, and David L. Rimm

9) Clinical Cancer Research 2001 7: 4013-4020.
Clinical Significance of Molecular Expression Profiles of Hurthle Cell Tumors of the Thyroid Gland Analyzed via Tissue Microarrays

Axel Hoos, Alexander Stojadinovic, Bhuvanesh Singh, Maria E. Dudas, Denis H. Y. Leung, Ashok R. Shaha, Jatin P. Shah, Murray F. Brennan, Carlos Cordon-Cardo, and Ronald Ghossein

10) American Journal of Pathology 2002 160: 175-183.
Reduction of Hematopoietic Cell-Specific Tyrosine Phosphatase SHP-1 Gene Expression in Natural Killer Cell Lymphoma and Various Types of Lymphomas/Leukemias : Combination Analysis with cDNA Expression Array and Tissue Microarray

Takashi Oka, Tadashi Yoshino, Kazuhiko Hayashi, Nobuya Ohara, Tohru Nakanishi, Yuichiro Yamaai, Akio Hiraki, Chiharu Aoki Sogawa, Eisaku Kondo, Norihiro Teramoto, Kiyoshi Takahashi, Junjiro Tsuchiyama, and Tadaatsu Akagi
 
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