◈ 응용분야
Tissue microarray 기술은 J. Kononen 등에 의해 (Nature Medicine Vol 4. No. 7, 1998 pp. 844-847) 발표되었으며, 다양한 연구분야에 종사하는 연구그룹으로부터 상당한 관심을 끌어 왔습니다. 이 기술은 자체적으로 다른 대용량 실험방법, 즉 DNA microarray, protein array 등과 여러가지 면에서 흡사합니다. 타겟 유효성확인 과정을 단기화하거나 치료용 항체의 교차 반응 체크 등을 매우 짧은 시간에 실행해 냄으로써 신약개발과 같은 분야에서 그 생산성을 극대화 할 수 있습니다. 이러한 상업적인 잇점 이외에, 학술적인 연구자들도 자신의 연구 수행을 여러가지 기초 생명과학 분야와 의학 분야에서 향상시킬 수 있습니다.

→ Typical examples of possible applications
타겟의 유효성 확인 면역조직검사를 통한 단백질 분석

 

 타겟의 발현 양상 분석 1),2),7) * 각 정상조직에서
* 여러가지 유사질병에서 6), 7)
*암화과정 전반에 걸친 변화 2),4),5),10)
*종양의 전이과정
동물실험이나 세포주 실험에서의 결과를 인체 조직에서 확인

 
FISH 를 통한DNA검사 3)

 
ISH를 통한 RNA검사

 
치료용 후보물질의 전임상 개발과정 후보 항체의 교차반응 검사

 



1) American Journal of Pathology 2001 158: 1335-1344.
Expression and Potential Role of Fas-Associated Phosphatase-1 in Ovarian Cancer

Ivo Meinhold-Heerlein, Frank Stenner-Liewen, Heike Liewen, Shinichi Kitada, Maryla Krajewska, Stanislaw Krajewski, Juan M. Zapata, Anne Monks, Dominic A. Scudiero, Thomas Bauknecht, and John C. Reed

2) The American Journal of Surgical Pathology 2002 26:312-319
Tissue Microarray Sampling Strategy for Prostate Cancer Biomarker Analysis

Mark A. Rubin, M.D.; Rodney Dunn, M.S.; Myla Strawderman, M.S.; Kenneth J. Pienta, M.D.

3) Cancer Research 2002 62: 1256-1260.
Clinical Validation of Candidate Genes Associated with Prostate Cancer Progression in the CWR22 Model System using Tissue Microarrays

Spyro Mousses, Lukas Bubendorf, Urs Wagner, Galen Hostetter, Juha Kononen, Robert Cornelison, Natalie Goldberger, Abdel G. Elkahloun, Niels Willi, Pasi Koivisto, William Ferhle, Mark Raffeld, Guito Sauter, and Olli-P Kallioniemi


4) Cancer Research 1999 59: 803-806.
High-Throughput Tissue Microarray Analysis of 3p25 (RAF1) and 8p12 (FGFR1) Copy Number Alterations in Urinary Bladder Cancer

Ronald Simon, Jan Richter, Urs Wagner, Andre Fijan, James Bruderer, Ulrico Schmid, Daniel Ackermann, Robert Maurer, Goran Alund, Hartmut Knonagel, Marcus Rist, Kim Wilber, Manuel Anabitarte, Franz Hering, Thomas Hardmeier, Andreas Schonenberger, Renata Flury, Peter Jager, Jean Luc Fehr, Peter Schraml, Holger Moch, Michael J. Mihatsch, Thomas Gasser, and Guido Sauter

5) Cancer Research 2001 61: 4514-4519.
High-Throughput Tissue Microarray Analysis of Cyclin E Gene Amplification and Overexpression in Urinary Bladder Cancer

Jan Richter, Urs Wagner, Juha Kononen, Andre Fijan, James Bruderer, Ulrico Schmid, Daniel Ackermann, Robert Maurer, Goran Alund, Hartmut Knonagel, Marcus Rist, Kim Wilber, Manuel Anabitarte, Franz Hering, Thomas Hardmeier, Andreas Schonenberger, Renata Flury, Peter Jager, Jean Luc Fehr, Peter Schraml, Holger Moch, Michael J. Mihatsch, Thomas Gasser, Olli P. Kallioniemi, and Guido Sauter

6) The Journal of Pathology 2001 194 (3): 349-357
Microarrays of bladder cancer tissue are highly representative of proliferation index and histological grade

Antonio Nocito, Lukas Bubendorf, Eva Maria Tinner, Katrin Suess, Urs Wagner, Thomas Forster, Juha Kononen, Andre Fijan, James Bruderer, Ulrico Schmid, Daniel Ackermann, Robert Maurer, Goran Alund, Hartmut Knonagel, Marcus Rist, Manuel Anabitarte, Franz Hering, Thomas Hardmeier, Andreas J. Schoenenberger, Renata Flury, Peter Jager, Jean Luc Fehr, Peter Schraml, Holger Moch, Michael J. Mihatsch, Thomas Gasser, Guido Sauter

7) American Journal of Pathology 1999 154: 981-986.
Identification of Differentially Expressed Genes in Human Gliomas by DNA Microarray and Tissue Chip Techniques

Satu-Leena Sallinen, Pauli K. Sallinen, Hannu K. Haapasalo, Heikki J. Helin, Pauli T. Helen, Peter Schraml, Olli-P. Kallioniemi, and Juha Kononen

8) Applied Immunohistochemistry & Molecular Morphology 2001 9:358-363
Tissue Microarray Analysis of ß-Catenin in Colorectal Cancer Shows Nuclear Phospho-ß-catenin Is Associated with a Better Prognosis

Gina G. Chung, Elayne Provost, Eric P. Kielhorn, Lori A. Charette, Bradley L. Smith, and David L. Rimm

9) Clinical Cancer Research 2001 7: 4013-4020.
Clinical Significance of Molecular Expression Profiles of Hurthle Cell Tumors of the Thyroid Gland Analyzed via Tissue Microarrays

Axel Hoos, Alexander Stojadinovic, Bhuvanesh Singh, Maria E. Dudas, Denis H. Y. Leung, Ashok R. Shaha, Jatin P. Shah, Murray F. Brennan, Carlos Cordon-Cardo, and Ronald Ghossein

10) American Journal of Pathology 2002 160: 175-183.
Reduction of Hematopoietic Cell-Specific Tyrosine Phosphatase SHP-1 Gene Expression in Natural Killer Cell Lymphoma and Various Types of Lymphomas/Leukemias : Combination Analysis with cDNA Expression Array and Tissue Microarray

Takashi Oka, Tadashi Yoshino, Kazuhiko Hayashi, Nobuya Ohara, Tohru Nakanishi, Yuichiro Yamaai, Akio Hiraki, Chiharu Aoki Sogawa, Eisaku Kondo, Norihiro Teramoto, Kiyoshi Takahashi, Junjiro Tsuchiyama, and Tadaatsu Akagi